The influence of fusion tags to produce recombinant proteins in the cytoplasm of is not well-studied as in . This study aimed to investigate the influence of His-tags with different codons on the protein production levels of the high expression gene () and low expression gene () in the cytoplasm of cells. We used three different N-terminal His-tags, M-6xHis, MRGS-8xHis and MEA-8xHis, to investigate their effects on the production levels of GFP variants under the control of the P212 in . The fusions of His-tags with GFP+ caused a reduction compared to the construct without His-tag. When three His-tags fused with , the EGFP production levels were significantly increased up to 3.5-, 12-, and 15-fold. This study suggested that His-tag at the N-terminus could enhance the protein production for the low expression gene and reduce that of the high expression gene in .
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9326129 | PMC |
| http://dx.doi.org/10.1016/j.btre.2022.e00754 | DOI Listing |
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