The lytic spectrum of phages is usually limited to only a few strains of the same bacterial species that can lyse. In clinical molecular epidemiology, bacterial strains are commonly classified into sequence types (STs) using the multilocus sequence typing (MLST) approach. The aim of this study was to determine the association between the phage lytic spectrum and STs. MLST analysis of 11 extended-spectrum β-lactamase (ESBL)-producing Escherichia coli clinical isolates revealed that most belonged to ST73 or ST131, with four isolates each. Phages were isolated from sewage samples using various E. coli strains as hosts. The relationship between phage lytic spectra of ESBL-producing E. coli isolates ST73 and ST131 and STs was evaluated using Fisher's exact test. The lytic spectra of phages were significantly dependent on the ST classification of ST73 or ST131, suggesting that a phage lysing an isolate belonging to a particular ST could lyse other isolates of the same ST. We successfully isolated wide-host-range phages lysing all clinical isolates belonging to two clinically important ST types (ST73 and ST131).
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.7883/yoken.JJID.2022.206 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Prevalence and virulence profiles of ESBL-producing Escherichia coli in urinary and blood infections in South Korea.
Folia Microbiol (Praha)
October 2024
Department of Microbiology, College of Medicine, Gyeongsang National University, Jinju, 52727, Republic of Korea.
Article Synopsis
- ESBL-producing E. coli is a serious concern for extraintestinal infections due to its high antibiotic resistance, with a study done on isolates from urine and blood samples at three South Korean hospitals.
- The research found that CTX-M and TEM are the most common ESBL types present in a majority of isolates, and identified multiple high-risk E. coli clones linked to severe infections.
- The results also showed a significant presence of virulence factors in these strains, underlining the challenges they pose, especially for vulnerable populations, and emphasizing the need for improved infection control measures.
Phylogenetic group, antibiotic resistance, virulence gene, and genetic diversity of causing bloodstream infections in Iran.
Front Microbiol
July 2024
Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
is one of the most important pathogens causing bloodstream infections (BSIs) throughout the world. We sought to characterize the phylogroup classification, major human sequence types (STs), antimicrobial resistance, presence of selected antimicrobial resistance and virulence genes, and genetic diversity of isolated from patients with BSIs at the University Hospital in Iran. A total of 100 bloodstream isolates were collected between December 2020 and June 2022.
View Article and Find Full Text PDFTherapeutic Phage Candidates for Targeting Prevalent Sequence Types of Carbapenem-Resistant .
Foodborne Pathog Dis
November 2024
Department of Biomedical Science, School of Medicine, Kyungpook National University, Daegu, Republic of Korea.
Carbapenem-resistant (CREC) is a global threat to public health; therefore, alternative treatment options are urgently needed. Bacteriophages have emerged as promising candidates for combating CREC infections. This study aimed to investigate the genetic basis of phage sensitivity in CREC by evaluating carbapenem resistance among multidrug-resistant (MDR) isolated in Daegu, South Korea and analyzing their sequence types (STs) with phage susceptibility spectra.
View Article and Find Full Text PDFA microbiological and genomic perspective of globally collected Escherichia coli from adults hospitalized with invasive E. coli disease.
J Antimicrob Chemother
September 2024
Laboratory of Medical Microbiology, Vaccine & Infectious Disease Institute, University of Antwerp, Antwerp, Belgium.
Objectives: Escherichia coli can cause infections in the urinary tract and in normally sterile body sites leading to invasive E. coli disease (IED), including bacteraemia and sepsis, with older populations at increased risk. We aimed to estimate the theoretical coverage rate by the ExPEC4V and 9V vaccine candidates.
View Article and Find Full Text PDFDistribution of and Variants among Genotypes: Association with Major Extraintestinal Pathogenic Lineages.
Int J Mol Sci
June 2024
Programa de Microbiología y Micología, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Av. Independencia 1027, Santiago 8380453, Chile.
The pyelonephritis-associated fimbria (P fimbria) is one of the most recognized adhesion determinants of extraintestinal pathogenic strains (ExPECs). Twelve variants have been described for the gene encoding the P fimbria major structural subunit PapA and three variants for the gene encoding the adhesin subunit PapG. However, their distribution among the ExPEC diversity has not been comprehensively addressed.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!