Photodegradation greatly affects everyday life. It poses challenges when food deteriorates or when objects of cultural heritage fade, but it can also create opportunities applied in advanced oxidation processes in water purification. Studying photodegradation, however, can be difficult because of the time needed for degradation, the inaccessibility of pure compounds, and the need to handle samples manually. A novel light-exposure cell, based on liquid-core-waveguide (LCW) technology, was embedded in a multiple-heart-cut two-dimensional liquid chromatography system by coupling the LCW cell to the multiple-heart-cut valve. The sample was flushed from the heart-cut loops into the cell by an isocratic pump. Samples were then irradiated using different time intervals and subsequently transferred by the same isocratic pump to a second-dimension sample loop. The mixture containing the transformation products was then subjected to the second-dimension separation. In the current setup, about 30-40% of the selected fraction was transferred. Multiple degradation products could be monitored. Degradation was found to be faster when a smaller sample amount was introduced (0.3 μg as compared to 1.5 μg). The system was tested with three applications, that is, fuchsin, a 19th-century synthetic organic colorant, annatto, a lipophilic food dye, and vitamin B complex.
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http://dx.doi.org/10.1021/acs.analchem.2c01928 | DOI Listing |
Anal Chem
August 2022
van 't Hoff Institute for Molecular Sciences, Analytical-Chemistry Group, University of Amsterdam, Science Park 904, 1098 XH Amsterdam, The Netherlands.
Photodegradation greatly affects everyday life. It poses challenges when food deteriorates or when objects of cultural heritage fade, but it can also create opportunities applied in advanced oxidation processes in water purification. Studying photodegradation, however, can be difficult because of the time needed for degradation, the inaccessibility of pure compounds, and the need to handle samples manually.
View Article and Find Full Text PDFJ Chromatogr A
October 2021
University of Münster, Institute of Inorganic and Analytical Chemistry, Corrensstraße 30, 48149 Münster, Germany. Electronic address:
Sphingolipids exert important functions in cells, ranging from stabilising the cell membrane to bioactive signalling in signal transduction pathways. Changed concentrations of sphingolipids are associated with, among others, neurodegenerative and cardiovascular diseases. In this work, we present a novel two-dimensional liquid chromatography method (2D-LC) coupled to tandem mass spectrometry (MS/MS) for the identification of ceramides, hexosylceramides and sphingomyelins in the model organism Caenorhabditis elegans (C.
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