AI Article Synopsis
- The cilium is a crucial organelle responsible for various cellular functions such as movement, sensing, and signaling, with a specialized base that controls the import of transport trains carrying essential proteins.
- This research utilized in situ cryo-electron tomography to explore the transition zone and the assembly process of intraflagellar transport (IFT) trains at the ciliary base.
- The study outlined a stepwise assembly process for IFT trains, detailing how IFT-B forms the backbone followed by the addition of IFT-A, dynein-1b, and kinesin-2 before they enter the cilium.
Article Abstract
The cilium is an antenna-like organelle that performs numerous cellular functions, including motility, sensing, and signaling. The base of the cilium contains a selective barrier that regulates the entry of large intraflagellar transport (IFT) trains, which carry cargo proteins required for ciliary assembly and maintenance. However, the native architecture of the ciliary base and the process of IFT train assembly remain unresolved. In this work, we used in situ cryo-electron tomography to reveal native structures of the transition zone region and assembling IFT trains at the ciliary base in . We combined this direct cellular visualization with ultrastructure expansion microscopy to describe the front-to-back stepwise assembly of IFT trains: IFT-B forms the backbone, onto which bind IFT-A, dynein-1b, and finally kinesin-2 before entry into the cilium.
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| http://dx.doi.org/10.1126/science.abm6704 | DOI Listing |
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