Eurytrematosis is a disease caused by flukes of the genus . These parasites infect the pancreatic ducts of a wide variety of species, including cattle, sheep and humans. Diagnosing eurytrematosis through the analysis of faecal samples can be difficult because most of the available techniques are considered of low sensitivity. In this context, a modification of the Dennis, Stone and Swanson technique (Belem Sedimentation Technique, BST) was previously developed to increase the probability of detecting infected animals; nevertheless, the values of eggs per gram obtained using the modified technique are generally low. We proposed a modification of the this technique (MBST), to increase the sensitivity and detection rate of infected animals. The objective of this work was to describe MBST and compare it with BST. Faecal samples of 212 clinically healthy animals (174 from cattle and 38 from sheep) from 20 farms were taken by the intra-rectal route and stored at 4°C. The samples were processed using BST and MBST. Positive samples amounted to 55 (25.9%) using BST and 121 (57.1%) using MBST. In the simples from cattle, 52 (29.8%) and 107 (61.4%) were positive in BST and MBST, respectively. In sheep, three (7.8%) and 14 (36.8%) positive samples were obtained in BST and MBST, respectively.The results obtained using the two methods were significantly different, indicating a lack of agreement between their findings. The results suggest that MBST is a more sensitive method to detect in faeces than BST.
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http://dx.doi.org/10.1017/S0022149X22000414 | DOI Listing |
Brief Bioinform
November 2024
State Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Yuanmingyuan West Road, Beijing, 100193, China.
Nonadditive genetic effects pose significant challenges to traditional genomic selection methods for quantitative traits. Machine learning approaches, particularly kernel-based methods, offer promising solutions to overcome these limitations. In this study, we developed a novel machine learning method, KPRR, which integrated a polynomial kernel into ridge regression to effectively capture nonadditive genetic effects.
View Article and Find Full Text PDFAppl Environ Microbiol
December 2024
Microbiological Sciences Department, North Dakota State University, Fargo, North Dakota, USA.
J Environ Manage
December 2024
Department of Grassland Science, College of Grassland Science & Technology, Sichuan Agricultural University, No.211 Huimin Road, Wenjiang District, Chengdu, 611130, China.
Arbuscular mycorrhizal fungi (AMF) form extensive symbiotic relationships with plants, which are critical for plant-driven biogeochemical cycles and ecosystem functions. Grazing and mowing, which are common grassland utilization patterns globally, significantly alter plant community characteristics as well as soil nutrients and structure, thereby potentially influencing AMF communities. However, the effects of these grassland managements on AMF community structure and ecological processes remain unclear.
View Article and Find Full Text PDFAm J Trop Med Hyg
December 2024
Department of Infectious Diseases, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Republic of Korea.
Vet Parasitol
December 2024
Department of Medicine, Faculty of Veterinary Medicine and Animal Science, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur 1706, Bangladesh. Electronic address:
Blastocystis is a ubiquitous gastrointestinal protozoan parasite found both in humans and animals. The purpose of this review is to look at the prevalence and genetic diversity of Blastocystis in farm animals, including cattle, sheep, goats, pigs, and poultry, and discuss the potential evidence of transmission between animals and humans, as well as highlight the related risk factors and public health significance. Significant differences have been found in the prevalence of Blastocystis in different hosts worldwide.
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