Preliminary Study on Gene Regulation and its Pathways in Chinese Holstein Cows with Clinical Mastitis Caused by .

Introduction: Clinical mastitis (CM) is one of the most common diseases of dairy cows globally, has a complex aetiology and recurs easily. is a frequently isolated pathogen responsible for bovine mastitis and remains difficult to eradicate.

Material And Methods: To characterise the transcriptional profiles of dairy cows infected by , we performed an RNA-seq analysis of peripheral blood leukocytes in lactating Chinese Holstein dairy cows with CM and did the same with healthy cows' samples as controls.

Results: A total of 4,286 genes were detected in the CM cases infected with which were differentially expressed compared to the controls, 3,085 of which were upregulated, the remainder being downregulated. Notably, we observed that some differentially expressed genes (DEGs) had strong protein-protein interaction. Of these, six downregulated DEGs (, , , , , and ) were involved in the metabolic pathway, while eight upregulated DEGs (, , , , , , , and ) were associated with the platelet activation pathway.

Conclusion: The transcriptome dataset of CM cases would be a valuable resource for clinical guidance on anti-inflammatory medication and for deeper understanding of the biological processes of CM response to infection, and it would enable us to identify specific genes for diagnostic markers and possibly for targeted therapy.