Molecular detection of Aspergilli from commercial chicken in selected areas of Bangladesh.

Objectives: This study was designed to isolate, identify, and determine the prevalence of in commercial chicken in selected areas of Bangladesh.

Materials And Methods: A total of 50 lung samples from suspected dead chickens, comprising broilers ( = 32) and layers ( = 18), aged between 5 days and 45 weeks, were collected from poultry farms located in the Gazipur district in Bangladesh. Fungi were primarily identified based on the colony morphology using potato dextrose agar (PDA). DNA was extracted from the suspected colonies. spp. was detected by genus-specific ASAP-1 and ASAP-2. spp. were then screened by polymerase chain reaction targeting (FLA-1 and FLA-2), (ASPU and Af3r), and (ASPU and Nilr).

Results: The overall prevalence of spp. was 44% ( = 22/50; 0.05). Among the , was detected in 10% ( = 5/50) of the samples. Similarly, and were detected at 26% ( = 13/50) and 8% ( = 4/50) respectively. Three samples were associated with more than one fungus; two fungi ( and . ) were in two samples, and three fungi (, , and ) were in one sample.

Conclusion: Isolation and prevalence of spp. in commercial chicken were studied for the first time in Bangladesh.