The aim of this study was to highlight antimicrobial resistance among isolated from bloodstream infections in hospitals in southern Poland. The present study includes laboratory-confirmed secondary bloodstream infections (LC-BSIs), in the years 2015-2018, in hospitalized adult patients (≥18). Episodes of BSIs were defined according to the strictly described guidelines. Antimicrobial susceptibility testing was performed with the automated system and the disc diffusion method. Extended-spectrum β-lactamases (ESBLs)-producing were detected using the double-disc synergy test. Between 2015 and 2018, 356 episodes of secondary BSIs in 997 patients aged 21-96 years were documented in a prospective study, including 134 (37.6%) ESBL-producing . was the predominant pathogen in internal medicine (37.6%) and surgery units (46.8%); in intensive care units (ICUs), was isolated more frequently (33.3%). were highly resistant to most antimicrobial agents. isolates had a higher level of resistance than , regardless of the unit. The increase in AMR and the widespread distribution of ESBL-producing in Polish hospitals can be related to the lack of or inappropriate antibiotic treatment.
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http://dx.doi.org/10.3390/jcm11143927 | DOI Listing |
Background And Aims: A catheter-related bloodstream infection (CRBSI) is a life-threatening complication of hemodialysis. It is responsible for significant morbidity and mortality and a costly long hospital stay. Despite its burden, little is known about the factors associated with it and the antibiogram of its responsible causative bacteria.
View Article and Find Full Text PDFInfect Prev Pract
March 2025
Cardio Thoracic and Vascular Surgery, PGIMER, Chandigarh, India.
Background: Infection prevention and control (IPC) practices by critical care nurses are crucial in preventing ventilator-associated pneumonia (VAP) and central-line-associated bloodstream infection (CLABSI).
Aim: To implement an integrative approach to developing a set of IPC practices and disseminating information on the IPC practices through an educational multimedia tool to improve compliance with the practices.
Methods: This participatory interventional before-after study was conducted in a single tertiary care centre's cardiac surgical intensive care unit (ICU) from May 2022 to March 2023.
Acta Pharm Sin B
December 2024
Department of Molecular and Life Science, Hanyang University, Ansan 15588, Republic of Korea.
The pathophysiology of sepsis is characterized by a systemic inflammatory response to infection; however, the cytokine blockade that targets a specific early inflammatory mediator, such as tumor necrosis factor, has shown disappointing results in clinical trials. During sepsis, excessive endotoxins are internalized into the cytoplasm of immune cells, resulting in dysregulated pyroptotic cell death, which induces the leakage of late mediator alarmins such as HMGB1 and PTX3. As late mediators of lethal sepsis, overwhelming amounts of alarmins bind to high-affinity TLR4/MD2 and low-affinity RAGE receptors, thereby amplifying inflammation during early-stage sepsis.
View Article and Find Full Text PDFInfect Drug Resist
January 2025
Department of Critical Care Medicine, Lanzhou University Second Hospital, Lanzhou University, Lanzhou City, Gansu Province, People's Republic of China.
Aim: Sepsis is a potentially fatal condition characterized by organ failure resulting from an abnormal host response to infection, often leading to liver and kidney damage. Timely recognition and intervention of these dysfunctions have the potential to significantly reduce sepsis mortality rates. Recent studies have emphasized the critical role of serum exosomes and their miRNA content in mediating sepsis-induced organ dysfunction.
View Article and Find Full Text PDFAPMIS
January 2025
Department of Laboratory Medicine, Clinical Microbiology Örebro University Hospital and Faculty of Medicine and Health at Örebro University, Örebro, Sweden.
Shotgun metagenomics offers a broad detection of pathogens for rapid blood stream infection of pathogens but struggles with often low numbers of pathogens combined with high levels of human background DNA in clinical samples. This study aimed to develop a shotgun metagenomics protocol using blood spiked with various bacteria and to assess bacterial DNA extraction efficiency with human DNA depletion. The Blood Pathogen Kit (Molzym) was used to extract DNA from EDTA-whole blood (WB) and plasma samples, using contrived blood specimens spiked with bacteria for shotgun metagenomics diagnostics via Oxford Nanopore sequencing and PCR-based library preparation.
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