The Gly-Asp-Ser-Leu (GDSL)-lipase family is a large subfamily of lipolytic enzymes that plays an important role in plant growth and defense against environmental stress. However, little is known about their function in pecans ( K. Koch). In this study, 87 s were identified and divided into 2 groups and 12 subgroups using phylogenetic analysis; members of the same sub-branch had conserved gene structure and motif composition. The majority of the genes had four introns and were composed of an α-helix and a β-strand. Subcellular localization analysis revealed that these genes were localized in the extracellular matrix, chloroplasts, cytoplasm, nucleus, vacuole, and endoplasmic reticulum, and were validated by transient expression in tobacco mesophyll cells. Furthermore, the analysis of the promoter -elements for the s revealed the presence of plant anaerobic induction regulatory, abscisic acid response, light response elements, jasmonic acid (JA) response elements, etc. The qRT-PCR analysis results in "Pawnee" with salt treatment showed that the (leaf) and (root) were highly expressed in different tissues. After salt stress treatment, isobaric tags for relative and absolute quantitation (iTRAQ) analysis revealed the presence of a total of ten GDSL proteins. Moreover, the weighted gene co-expression network analysis (WGCNA) showed that one set of co-expressed genes (module), primarily , , and was significantly associated with salt stress in leaf. In short, some of them were shown to be involved in plant defense against salt stress in this study.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9323844PMC
http://dx.doi.org/10.3390/genes13071103DOI Listing

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  • This text indicates that there is a correction to the article with DOI: 10.1371/journal.pone.0294573.
  • It suggests that some errors or inaccuracies in the original publication have been addressed.
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