AI Article Synopsis
- * Whole-genome sequencing of select isolates revealed various insertion sequences that enhance the ability to capture and spread antibiotic resistance genes, particularly those associated with OXA-23, OXA-66, and OXA-82 oxacillinases.
- * The multi-locus sequence typing indicated that all examined strains belong to the same international clone, known as sequence type 2 (ST), which is linked with increased virulence due to various pathogenic factors.
Article Abstract
A total of 43 strains, isolated from 43 patients affected by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and by bacterial sepsis, were analyzed by antimicrobial susceptibility testing. All strains were resistant to almost three different classes of antibiotics, including carbapenems and colistin. The whole-genome sequencing (WGS) of eight selected isolates showed the presence of different insertion sequences (ISs), such as IS, IS, IS, IS, IS, IS and IS giving to a high ability to capture and mobilize antibiotic resistance genes. Resistance to carbapenems is mainly mediated by the presence of OXA-23, OXA-66 and OXA-82 oxacillinases belonging to OXA-51-like enzymes. The presence of AmpC cephalosporinase, ADC-25, was identified in all . The pathogenicity of was exacerbated by the presence of several virulence factors. The multi-locus sequence typing (MLST) analysis showed that all strains belong to sequence type 2 (ST) international clone.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9311945 | PMC |
| http://dx.doi.org/10.3390/antibiotics11070955 | DOI Listing |
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