Therapeutic Effect and Immune Changes after Treatment of -Infected BALB/c Mice with Compounds Isolated from .

Background/purpose: is globally distributed. has been studied as a treatment, including the nematodes and protozoa, but no research results are related to cestodes. Therefore, the aim of this study was to target .

Methods: The natural components of were isolated and added to which was cultured in vitro, to observe changes in the mortality, motility, and morphology. BALB/c male mice infected with were treated with effective components of for 10 days, and the changes were recorded. After the mice were sacrificed, the spleen weight was measured, and a primary culture was performed for the subsequent cytokine and chemokine testing.

Results: The experiment found that 13-hydroxy-(13-S)-pheophytin a and aristophyll-C have clear cestocidal effects in vitro. 13-hydroxy-(13-S)-pheophytin a has been shown to be effective at reducing parasite populations and eliciting host immune responses in vivo. IL-2, IL-4, IL-5, IL-6, IL-10, IL-13, IL-17, MCP-1, IFN-γ, TNF-α, MIP-1α, and GM-CSF in 13-hydroxy-(13-S)-pheophytin a were significantly increased after stimulation, while IL-1α, IL-1β, IL-3, IL-12p70, and RANTES were unchanged.

Conclusions: The investigation shows that components of have actual cestocidal activity against .