Background: Critical bone defects are a significant problem in clinics. The periosteum plays a vital role in bone regeneration. A tissue-engineered periosteum (TEP) has received increasing attention as a novel strategy for bone defect repairs.
Methods: In this experiment, a biomimetic periosteum was fabricated by using coaxial electrospinning technology with decellularized porcine small intestinal submucosa (SIS) as the shell and polycaprolactone (PCL) as the core. In vitro, the effects of the biomimetic periosteum on Schwann cells, vascular endothelial cells, and bone marrow mesenchymal stem cells were detected by a scratch test, an EdU, a tube-forming test, and an osteogenesis test. In vivo, we used HE staining to evaluate the effect of the biomimetic periosteum on bone regeneration.
Results: In vitro experiments showed that the biomimetic periosteum could significantly promote the formation of angiogenesis, osteogenesis, and repaired Schwann cells (SCs). In vivo experiments showed that the biomimetic periosteum could promote the repair of bone defects.
Conclusions: The biomimetic periosteum could simulate the structural function of the periosteum and promote bone repair. This strategy may provide a promising method for the clinical treatment of skull bone defects.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9323854 | PMC |
| http://dx.doi.org/10.3390/membranes12070719 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
A self-forming bone membrane generated by periosteum-derived stem cell spheroids enhances the repair of bone defects.
Acta Biomater
December 2024
Department of Orthopedics, Shenzhen Hospital, Southern Medical University, Shenzhen 518000, PR China; The Third School of Clinical Medicine, Southern Medical University, Guangzhou 510000, PR China. Electronic address:
The periosteum, a highly specialized thin tissue, is instrumental in contributing to as much as 70% of early bone formation. Recognizing the periosteum's vital physiological roles, the fabrication of a biomimetic periosteum has risen as an auspicious strategy for addressing extensive bone defects. In the study, we obtained such biomimetic periosteum by utilizing periosteum-derived stem cells (PDSCs) spheroids.
View Article and Find Full Text PDFFast bone regeneration using injectable fully biomimetic organoids with biomineralized and organic microenvironments.
Biomater Sci
December 2024
Bone Trauma Surgery, The 960th Hospital of the PLA Joint Logistics Support Force, Jinan, 250031, China.
Bone defects and congenital bone deficiencies are common clinical conditions. However, conventional non-degradable artificial materials often lead to serious complications, such as severe infections and material displacement. The emergence of tissue engineering and the organoid concept presents a promising approach for the repair of bone defects, facilitating physiological reconstruction while minimizing complications.
View Article and Find Full Text PDFConstruction of bilayer biomimetic periosteum based on SLA-3D printing for bone regeneration.
Colloids Surf B Biointerfaces
February 2025
Center for Advanced Jet Engineering Technologies (CaJET), School of Mechanical Engineering, Shandong University, Jinan 250061, China; Key Laboratory of High Efficiency and Clean Mechanical Manufacture, Ministry of Education, Shandong University, China; National Demonstration Center for Experimental Mechanical Engineering Education (Shandong University), China; Additive Manufacturing Research Center of Shandong University of National Engineering Research Center of Rapid Manufacturing, Jinan 250061, China.
An ideal biomimetic periosteum should have excellent biocompatibility to promote osteoclast adhesion and improve osseointegration, which is significant in promoting bone regeneration. In this work, a bionic artificial periosteum printed by the SLA-3D printing was prepared, consisting of a poly (ethylene glycol) diacrylate (PEGDA)/chitosan/tricalcium phosphate (TCP) fibrous layer and a gelatin methacryloyl (GelMA)/ammonium molybdate (Mo) cambium layer. Distinct surface characteristics were achieved on both sides of the biomimetic periosteum.
View Article and Find Full Text PDFA Bottom-Up Approach to Assemble Cell-Laden Biomineralized Nanofiber Mats into 3D Multilayer Periosteum Mimics for Bone Regeneration.
Nano Lett
November 2024
National Engineering Research Center for Biomaterials, Department of Biomedical Engineering, Sichuan University, Chengdu 610064, China.
The creation of complex multilayer periosteal graft structures is challenging. This study introduced a novel bottom-up approach to assemble cell-laden nanofiber mats into a three-dimensional (3D) multilayer periosteum mimic, successfully replicating the hierarchical complexity of the natural periosteum. These nanofiber mats, which were fabricated by electrospinning, surface modification, and stimulated body fluid (SBF) immersion, are composed of nanoscale polycaprolactone (PCL) fibers coated with a mineralized collagen layer along the fiber orientation.
View Article and Find Full Text PDFBiomimetic periosteum-bone scaffolds with codelivery of BMP-2 and PDGF-BB for skull repair.
Bone
January 2025
Department of Orthopedics, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215300, China. Electronic address:
Tissue engineering employs the use of bioactive materials to facilitate the filling and acceleration of bone defect healing, thereby introducing novel concepts to the field of in situ bone repair. Some studies have shown that periosteum plays an important role in bone regeneration and repair. In this study, biomimetic periosteum-bone scaffolds were prepared by depositing poly-L-lactic acid (PLLA) electrospun fibers on the surface of the gelatin/chitosan cryogel to mimic the bone and periosteum structure, respectively.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!