AI Article Synopsis
- The ongoing study of the SCSIO T05 genome has resulted in the discovery of new lipopeptides named autucedines A-C, marking the 10th skeleton identified from this strain.
- Researchers verified the related biosynthetic gene cluster through heterogeneous expression, leading to the isolation of two additional variants, autucedines D and E.
- Gene inactivation experiments indicated that the formation of the unique lipochain-linked dihydro-β-alanine unit occurs before the enzyme assembly process known as NRPS (non-ribosomal peptide synthetase) takes place.
Article Abstract
Continuously mining the SCSIO T05 genome leads to the identification of new lipopeptides (autucedines A-C), constituting members of the 10th skeleton isolated from this strain. The corresponding biosynthetic gene cluster () was verified by heterogeneous expression, and another two analogues (autucedines D and E) were isolated from the heterogeneous expression strain. Gene inactivation experiments revealed that construction of the unique "lipochain-linked dihydro-β-alanine" unit takes place prior to the NRPS assembly line.
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| http://dx.doi.org/10.1021/acs.orglett.2c01957 | DOI Listing |
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