AI Article Synopsis
- - A new monoclonal antibody was created specifically for detecting tyramine, utilizing a novel hapten design.
- - Researchers developed a smartphone-assisted multicolor immunoassay that uses enzyme-driven color changes for visual evaluation of tyramine levels.
- - The assay can accurately detect tyramine in food products like beef, pork, and yogurt at a sensitivity of 19.7 mg/kg, with validation results showing high reliability against traditional methods.
Article Abstract
In this work, a specific monoclonal antibody against tyramine was produced based on a new hapten design. Then, we developed a high-resolution multicolor colorimetric immunoassay for tyramine based on this antibody by integrating enzyme-induced multicolor generation with smartphone-assistant signal readout. The multicolor generation is due to the shift of the local surface plasmon resonance band of gold nanostructure controlled by alkaline phosphatase-induced the growth of gold nanostars. Quantitative detection of tyramine was achieved via analyzing the red/blue channel values of assay solution's image taken by a smartphone with the support of a color recognizer application. The limit of detection of this immunoassay for tyramine detection in beef, pork and yoghurt was 19.7 mg/kg or L. The average recoveries were between 83 % and 103 %., and the results were validated by high performance liquid chromatography to be reliable. Overall, this developed immunoassay provides a promising platform for on-site detection of tyramine.
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| http://dx.doi.org/10.1016/j.foodchem.2022.133729 | DOI Listing |
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