AI Article Synopsis
- The K98 capsular polysaccharide (CPS) from Acinetobacter baumannii clinical isolate REV-1184 was analyzed using various techniques, revealing its linear structure of tetrasaccharide repeats called K-units.
- These K-units consist of four unique sugar residues, including d-GlcpNAc and d-GalpNAc, with the latter modified by a pyruvic acid acetal group.
- Although K98 shares a similar composition with A. baumannii K4, the K98 CPS is linear while K4 is branched, attributed to differences in the Wzy polymerases responsible for their respective biosynthesis.
Article Abstract
The K98 capsular polysaccharide (CPS) from the Acinetobacter baumannii clinical isolate, REV-1184, was studied by sugar analysis and Smith degradation along with one- and two-dimensional H and C NMR spectroscopy and high-resolution electrospray ionization mass spectrometry. The CPS was found to consist of linear tetrasaccharide repeats (K-units) that include one residue each of d-GlcpNAc, d-GalpNAc, 2-acetamido-2-deoxy-d-galacturonic acid (d-GalpNAcA), and 2-acetamido-2,6-dideoxy-d-glucose (N-acetylquinovosamine, d-QuipNAc), with the GalpNAc residue decorated with a (R)-configurated 4,6-pyruvic acid acetal group. The CPS has a similar composition to that of A. baumannii K4 but the topology of the tetrasaccharide K-unit is different (linear in K98 versus branched in K4). This was due to a difference in sequence for the Wzy polymerases encoded by the CPS biosynthesis gene clusters KL98 and KL4, with the Wzy polymerase forming a β-d-QuipNAc-(1→3)-d-GalpNAc linkage between the K98 units.
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Structure of the K98 capsular polysaccharide from Acinetobacter baumannii REV-1184 containing a cyclic pyruvic acid acetal.
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Article Synopsis
- The K98 capsular polysaccharide (CPS) from Acinetobacter baumannii clinical isolate REV-1184 was analyzed using various techniques, revealing its linear structure of tetrasaccharide repeats called K-units.
- These K-units consist of four unique sugar residues, including d-GlcpNAc and d-GalpNAc, with the latter modified by a pyruvic acid acetal group.
- Although K98 shares a similar composition with A. baumannii K4, the K98 CPS is linear while K4 is branched, attributed to differences in the Wzy polymerases responsible for their respective biosynthesis.
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