This study developed a closed-circuit biorefinery process for full conversion of lignocellulose into ethanol, biogas and organic fertilizer with zero waste on a pilot scale. In the process, subcritical water pretreatment could effectively break the structure of wheat straw (WS), and ethanol was obtained from pretreated wheat straw (PWS) using two batches of simultaneous saccharification and fermentation (SSF). The pretreatment and ethanol fermentation wastes were reused for biogas and organic fertilizer production by anaerobic digestion (AD), whereas the pretreatment and ethanol conversion efficiency were reduced when supernatant after AD was recovered for next batch pretreatment. The yields of ethanol (0.08-0.09 g/g), biogas (0.05-0.10 L/g) and organic fertilizer (0.55-0.79 g/g) were demonstrated through mass balance. Furthermore, the hidden problems were exposed on pilot-scale conversion process, and several strategies were provided for optimizing the biorefinery process in the future.
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http://dx.doi.org/10.1016/j.envres.2022.113946 | DOI Listing |
Chembiochem
January 2025
Vilnius University: Vilniaus Universitetas, Life Sciences Center Institute of Biotechnology, Vilnius, LITHUANIA.
Enzyme functional analysis is a multifaceted process that can be used for various purposes, such as screening for specific activities, as well as developing, optimising, and validating processes or final products. Functional analysis methods are crucial for assessing enzyme performance and catalytic properties. Laccase, a well-known blue multi-copper oxidase, holds immense potential in diverse industries such as pharmaceuticals, paper and pulp, food and beverages, textiles, and biorefineries due to its clean oxidation process and versatility in handling a wide range of substrates.
View Article and Find Full Text PDFEnzyme Microb Technol
January 2025
Institute of Biotechnology and Genetic Engineering, Chulalongkorn University, Bangkok 10330, Thailand; Center of Excellence in Bioconversion and Bioseparation for Platform Chemical Production, Institute of Biotechnology and Genetic Engineering, Chulalongkorn University, Bangkok 10330, Thailand; Water Science and Technology for Sustainable Environment Research Unit, Chulalongkorn University, Bangkok 10330, Thailand. Electronic address:
Inducer-free expression systems are promising tools for biorefinery because they can reduce the reliance on inducers, reducing production costs and simplifying processes. Owing to their broad range of substrate structures and catalytic reactions, cytochrome P450s are promising biocatalysts to produce value-added compounds. However, unsuitable levels of cytochrome P450 expression could result in cell stress, affecting the efficiency of the biocatalyst.
View Article and Find Full Text PDFJ Colloid Interface Sci
January 2025
School of Chemistry and Chemical Engineering, Guangxi Key Laboratory of Petrochemical Resource Processing and Process Intensification Technology, Guangxi University (GXU), 100 Daxuedong Road, Xixiangtang District, Nanning 530004 China. Electronic address:
Porous carbons with large surface area (>3000 m/g) and heteroatom dopants have shown great promise as electrode materials for zinc ion hybrid capacitors. Centralized mesopores are effective to accelerate kinetics, and edge nitrogen can efficiently enhance pseudocapacitive capability. It is a great challenge to engineer centralized mesopores and edge nitrogen in large-surface-area porous carbons.
View Article and Find Full Text PDFInt J Mol Sci
January 2025
Laboratory of Foodomics, Institute of Food Science Research, CIAL, CSIC, Nicolás Cabrera 9, 28049 Madrid, Spain.
This study investigates the biorefinery approach to extracting blood-brain barrier (BBB)-permeable compounds from Labill. and L. for neuroprotective purposes.
View Article and Find Full Text PDFInt J Biol Macromol
January 2025
Beijing Key Laboratory of Lignocellulosic Chemistry, State Key Laboratory of Efficient Production of Forest Resources Beijing Forestry University, Beijing 100083, China. Electronic address:
To develop a green solvent for lignin dissolution, the most fundamental aspects of its mechanism must be elucidated. Understanding the thermodynamic behaviors is of significant importance for designing novel deep eutectic solvents (DESs) to dissolve lignin. Herein, the heat of dissolution of lignin in acidic, alkaline or neutral DESs was determined by high-precision solution microcalorimetry, and comprehensively investigated the effect of physicochemical properties of DESs on the heat of dissolution and solubility of lignin.
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