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Multimodal Mass Spectrometry Identifies a Conserved Protective Epitope in Streptolysin O.
Anal Chem
June 2024
Division of Infection Medicine, Department of Clinical Sciences, Faculty of Medicine, Lund University, Klinikgatan 32, 222 42 Lund, Sweden.
An important element of antibody-guided vaccine design is the use of neutralizing or opsonic monoclonal antibodies to define protective epitopes in their native three-dimensional conformation. Here, we demonstrate a multimodal mass spectrometry-based strategy for in-depth characterization of antigen-antibody complexes to enable the identification of protective epitopes using the cytolytic exotoxin Streptolysin O (SLO) from as a showcase. We first discovered a monoclonal antibody with an undisclosed sequence capable of neutralizing SLO-mediated cytolysis.
View Article and Find Full Text PDFProximity labeling-assisted click conjugation for electrochemical analysis of specific subpopulations in circulating extracellular vesicles.
Biosens Bioelectron
July 2024
Center for Molecular Recognition and Biosensing, Shanghai Engineering Research Center of Organ Repair, School of Life Sciences, Shanghai University, Shanghai, 200444, China; Joint International Research Laboratory of Biomaterials and Biotechnology in Organ Repair (Ministry of Education), Shanghai University, Shanghai, 200444, China. Electronic address:
Sensitive and accurate analysis of specific subpopulations in circulating extracellular vesicles (EVs) can provide a wealth of information for cancer diagnosis and management. Thus, we propose herein a new electrochemical biosensing method based on a proximity labeling-assisted click conjugation strategy. The method's core design is use of antibody-guided proximity labeling to equip target EVs with a large amount of alkyne groups, so that azide-tagged silver nanoparticles can be accurately loaded onto target EV surfaces, via click conjugation, to generate significant electrochemical responses.
View Article and Find Full Text PDFUrgency and necessity of Epstein-Barr virus prophylactic vaccines.
NPJ Vaccines
December 2022
State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Department of Experimental Research, Sun Yat-sen University Cancer Center, Sun Yat-sen University, Guangzhou, Guangdong, PR China.
Epstein-Barr virus (EBV), a γ-herpesvirus, is the first identified oncogenic virus, which establishes permanent infection in humans. EBV causes infectious mononucleosis and is also tightly linked to many malignant diseases. Various vaccine formulations underwent testing in different animals or in humans.
View Article and Find Full Text PDFAntibody guided activatable NIR photosensitizing system for fluorescently monitored photodynamic therapy with reduced side effects.
J Control Release
March 2022
Department of Chemical Sciences, the Faculty of Natural Sciences, Ariel University, Ariel 40700, Israel. Electronic address:
Photodynamic therapy (PDT) utilizing an organic dye (photosensitizer) capable of killing cancer cells in the body upon light irradiation is one of the promising non-invasive treatment modalities for many cancers. A known drawback of PDT is a side-effect caused by existing photosensitizers to organs due to insufficient specificity and accidental light exposure of a patient during the delivery of the photosensitizer in the bloodstream. To overcome this issue, we developed a novel antibody guided, activatable photosensitizing system, Ab-mIXCy-Ac, where the trastuzumab (Ab) is linked to the non-active (not phototoxic and not fluorescent) dye, mIXCy-Ac, that contains the hydroxyl group protected by acetyl (Ac).
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