Relative quantitation methods rely on the use of reference substances to determine the content of samples. The aim of this study was to compare 1-84 parathyroid hormone (PTH) standards from different manufacturers to the WHO international standard 95/646. CE and LC with UV detection were investigated as quick and inexpensive quantitation methods, with an emphasis on selectivity between intact 1-84 PTH and its oxidized forms. Both methods were fully validated according to ICH Q2R1. Moreover, method performance was also evaluated according to guidelines defining the maximum allowable measurement uncertainty (MU) of a biological parameter from its intraindividual variation (CV), as well as the proportion of that MU devoted to the reference material. This study highlighted the fact that some 1-84 PTH standards have a content that is actually twice as high as the one stated on the label, which was confirmed by an amino acid analysis investigation. Our approach offers a quick and inexpensive way to estimate the content of 1-84 PTH standards.
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http://dx.doi.org/10.1016/j.jpba.2022.114942 | DOI Listing |
Background: Apart from massive weight loss, metabolic and bariatric surgery, especially gastric bypass (Roux-en-Y gastric bypass [RYGB]), can cause nutritional deficiencies. Proton pump inhibitors (PPI), relatively often used after RYGB, are associated with reduced calcium absorption. We have studied the long-term impact of PPI upon calcium homeostasis among RYGB patients.
View Article and Find Full Text PDFBiomimetics (Basel)
January 2025
Institute of Philosophy, University of Rostock, 18051 Rostock, Germany.
Biomimetics aims to learn from living systems to develop innovative technical artefacts. As it transcends disciplinary boundaries and needs to integrate both biological and technological knowledge, a domain ontology for biomimetics would be highly desirable. So far, several terminological resources have been designed to support the biomimetic development process.
View Article and Find Full Text PDFBMC Endocr Disord
January 2025
Department of Epidemiology and Biostatistics, Isfahan University of Medical Sciences, Isfahan, Iran.
Background: Primary hyperparathyroidism (pHPT) is the third most common endocrine system disorder. Parathyroidectomy (PTx) is the gold standard of care in symptomatic patients. Patients who are not surgical candidates may benefit from percutaneous ethanol ablation, which is a minimally invasive procedure.
View Article and Find Full Text PDFJ Pharm Biomed Anal
January 2025
Department of Laboratory Medicine, The First Hospital of Jilin University, Changchun 130021, China. Electronic address:
Accurate measurement of serum parathyroid hormone (PTH) is crucial for diagnosing and managing endocrine and osteological diseases. Conventional immunoassay methods struggle with cross reactivity issues between full-length PTH and truncated fragments or post-translationally modified forms. Both the standardization of PTH assays and the peptide's stability are concerning.
View Article and Find Full Text PDFSci Rep
January 2025
Health Management Center, The First Hospital of Hebei Medical University, No.89 Donggang Road, Yuhua District, Shijiazhuang, 050000, Hebei Province, China.
This study delves into the impact of precise health management coupled with physical rehabilitation on bone biomarkers in elderly patients with osteoporosis. Two hundred and forty individuals diagnosed with senile osteoporosis were randomly assigned to either the observation group (precision health management group, n = 120) or the control group (routine health management group, n = 120). Patients in the control group received standard health care, while those in the observation group received personalized health care along with physical therapy.
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