Insights into mobile genetic elements and the role of conjugative plasmid in transferring aminoglycoside resistance in extensively drug-resistant AB329.

is a major cause of nosocomial infection, and the incidence of extensively drug-resistant (XDRAB) infections has dramatically increased worldwide. In this study, we aimed to explore the complete genome sequence of XDRAB 329, ST1166/98 (Oxford/Pasteur), which is an outbreak clone from a hospital in Thailand. Whole-genome sequencing (WGS) was performed using short-read Illumina and long-read PacBio sequencing, and a conjugation assay of its plasmid was performed. The complete genome sequence of AB329 revealed a circular chromosome 3,948,038 bp in length with 39% GC content. Antibiotic resistance genes (ARGs), including beta-lactam resistance ( , , , , aminoglycoside resistance (-Ia, (3″)-Ib, (6)-Id, A), tetracycline resistance ((B), (R)), macrolide resistance ((E), (E)), and efflux pumps, were found. Mobile genetic elements (MGEs) analysis of AB329 revealed two plasmids (pAB329a and pAB329b), three prophages, 19 genomic islands (GIs), and 33 insertion sequences (ISs). pAB329a is a small circular plasmid of 8,731 bp, and pAB329b is a megaplasmid of 82,120 bp. (3')-VIa was detected in pAB329b, and a major facilitator superfamily (MFS) transporter was detected in the prophage resistance island 4 (AbaR4) harboring tetracycline and aminoglycoside resistance was detected in the genome of AB329. pAB329b, which belongs to Rep-type GR6 (plasmid lineage LN_1), is a conjugative plasmid with the ability to transfer an aminoglycoside resistance gene to sodium azide-resistant This study provides insights into the features of the MGEs of XDRAB which are the main reservoir and source of dissemination of ARGs.