AI Article Synopsis
- Small open reading frames (smORFs) play critical roles in regulating cellular functions and encoding microproteins, but identifying them genome-wide has been difficult.
- Researchers identified 7,767 smORFs in various human cell types and tissues, revealing patterns that match known proteins and highlighting tissue-specific sequences.
- The study also integrated data from mass spectrometry, confirming 603 small peptides at the protein level and contributing to a detailed understanding of the translated human genome.
Article Abstract
Translated small open reading frames (smORFs) can have important regulatory roles and encode microproteins, yet their genome-wide identification has been challenging. We determined the ribosome locations across six primary human cell types and five tissues and detected 7,767 smORFs with translational profiles matching those of known proteins. The human genome was found to contain highly cell-type- and tissue-specific smORFs and a subset that encodes highly conserved amino acid sequences. Changes in the translational efficiency of upstream-encoded smORFs (uORFs) and the corresponding main ORFs predominantly occur in the same direction. Integration with 456 mass-spectrometry datasets confirms the presence of 603 small peptides at the protein level in humans and provides insights into the subcellular localization of these small proteins. This study provides a comprehensive atlas of high-confidence translated smORFs derived from primary human cells and tissues in order to provide a more complete understanding of the translated human genome.
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| http://dx.doi.org/10.1016/j.molcel.2022.06.023 | DOI Listing |
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