AI Article Synopsis
- Wheat crops are often affected by stripe rust caused by the fungus Puccinia striiformis f. sp. tritici (Pst), leading to significant losses.
- Researchers have identified the TaPsIPK1 gene in wheat that makes plants more susceptible to Pst by interacting with a fungal effector, PsSpg1, which enhances TaPsIPK1's activity and its location in the cell.
- CRISPR-Cas9 technology used to inactivate TaPsIPK1 shows that it can provide broad-spectrum resistance to stripe rust while maintaining vital agricultural traits, indicating its potential for developing durable disease resistance through genetic modifications.
Article Abstract
Wheat crops are frequently devastated by pandemic stripe rust caused by Puccinia striiformis f. sp. tritici (Pst). Here, we identify and characterize a wheat receptor-like cytoplasmic kinase gene, TaPsIPK1, that confers susceptibility to this pathogen. PsSpg1, a secreted fungal effector vital for Pst virulence, can bind TaPsIPK1, enhance its kinase activity, and promote its nuclear localization, where it phosphorylates the transcription factor TaCBF1d for gene regulation. The phosphorylation of TaCBF1d switches its transcriptional activity on the downstream genes. CRISPR-Cas9 inactivation of TaPsIPK1 in wheat confers broad-spectrum resistance against Pst without impacting important agronomic traits in two years of field tests. The disruption of TaPsIPK1 leads to immune priming without constitutive activation of defense responses. Taken together, TaPsIPK1 is a susceptibility gene known to be targeted by rust effectors, and it has great potential for developing durable resistance against rust by genetic modifications.
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| http://dx.doi.org/10.1016/j.cell.2022.06.027 | DOI Listing |
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