AI Article Synopsis
- Oxidative and hypoxic stresses contribute to the degeneration of motor neurons and skeletal muscles in ALS, particularly in mice models with the G93A-SOD1 mutation.
- Researchers utilized bioluminescent imaging techniques to monitor cellular responses in ALS model mice, offering insights into oxidative stress using OKD48 detectors.
- A novel BRET probe was developed to study the effects of hypoxic stress, providing a practical protocol for in vivo imaging to better understand these processes in ALS models.
Article Abstract
Oxidative and hypoxic stresses are associated with the degeneration of both motor neurons and skeletal muscles in amyotrophic lateral sclerosis (ALS). In vivo bioluminescent imaging is used to monitor cellular responses to oxidative and hypoxic stresses in living ALS model mice bearing G93A-human Cu/Zn superoxide dismutase (SOD1) longitudinally using the IVIS spectrum imaging system. Double transgenic mice bearing both Keap1-dependent oxidative stress detector No-48 (OKD48) and G93A-SOD1 are useful for in vivo imaging of oxidative stress in ALS. We developed a bioluminescence resonance energy transfer (BRET) probe that is regulated by HIF-1α-specific ubiquitin-proteasome system. G93A-SOD1 mice injected with the BRET probe are useful to investigate the spatiotemporal responses to hypoxic stress in ALS. In this chapter, we introduce a practical protocol of in vivo imaging of both oxidative and hypoxic stress in ALS model mice.
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| http://dx.doi.org/10.1007/978-1-0716-2473-9_22 | DOI Listing |
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