Genome editing technology has rapidly evolved to knock-out genes, create targeted genetic variation, install precise insertion/deletion and single nucleotide changes, and perform large-scale alteration. The flexible and multipurpose editing technologies have started playing a substantial role in the field of plant disease management. CRISPR-Cas has reduced many limitations of earlier technologies and emerged as a versatile toolbox for genome manipulation. This review summarizes the phenomenal progress of the use of the CRISPR toolkit in the field of plant pathology. CRISPR-Cas toolbox aids in the basic studies on host-pathogen interaction, in identifying virulence genes in pathogens, deciphering resistance and susceptibility factors in host plants, and engineering host genome for developing resistance. We extensively reviewed the successful genome editing applications for host plant resistance against a wide range of biotic factors, including viruses, fungi, oomycetes, bacteria, nematodes, insect pests, and parasitic plants. Recent use of CRISPR-Cas gene drive to suppress the population of pathogens and pests has also been discussed. Furthermore, we highlight exciting new uses of the CRISPR-Cas system as diagnostic tools, which rapidly detect pathogenic microorganism. This comprehensive yet concise review discusses innumerable strategies to reduce the burden of crop protection.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.plantsci.2022.111376 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Global meta-analysis shows action is needed to halt genetic diversity loss.
Nature
January 2025
International Union for the Conservation of Nature (IUCN) Conservation Genetics Specialist Group (CGSG), .
Mitigating loss of genetic diversity is a major global biodiversity challenge. To meet recent international commitments to maintain genetic diversity within species, we need to understand relationships between threats, conservation management and genetic diversity change. Here we conduct a global analysis of genetic diversity change via meta-analysis of all available temporal measures of genetic diversity from more than three decades of research.
View Article and Find Full Text PDFOptimized sequential model for superior classification of plant disease.
Sci Rep
January 2025
D. Y. Patil Agriculture and Technical University, Talsande, Maharashtra, India.
Indian agriculture is vital sector in the country's economy, providing employment and sustenance to millions of farmers. However, Plant diseases are a serious risk to crop yields and farmers' livelihoods. Traditional plant disease diagnosis methods rely heavily on human expertise, which can lead to inaccuracies due to the invisible nature of early disease symptoms and the labor-intensive process, making them inefficient for large-scale agricultural management.
View Article and Find Full Text PDFGenetically-encoded targeted protein degradation technology to remove endogenous condensation-prone proteins and improve crop performance.
Nat Commun
January 2025
State Key Laboratory of Hybrid Rice, Institute for Advanced Studies (IAS), Wuhan University, Wuhan, Hubei, China.
Effective modulation of gene expression in plants is achievable through tools like CRISPR and RNA interference, yet methods for directly modifying endogenous proteins remain lacking. Here, we identify the E3 ubiquitin ligase E3TCD1 and develope a Targeted Condensation-prone-protein Degradation (TCD) strategy. The X-E3TCD1 fusion protein acts as a genetically engineered degrader, selectively targeting endogenous proteins prone to condensation.
View Article and Find Full Text PDFChromosome-level genome assembly of the sweet potato rot nematode Ditylenchus destructor.
Sci Data
January 2025
Shaanxi Key Laboratory of Plant Nematology, Bio-Agriculture Institute of Shaanxi, Xi'an, China.
Ditylenchus destructor, commonly known as the potato rot nematode, is a significant plant-parasitic pathogen affecting over 120 plant species globally. Effective control measures for D. destructor are limited, underscoring the need a high-quality reference genome to understand its pathogenic mechanisms.
View Article and Find Full Text PDFThe Role of Gut Microbiota in Shaping Immune Responses in Tephritidae Fruit Fly and Prospective Implications for Management.
Neotrop Entomol
January 2025
State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Key Laboratory of Bio Pesticide and Chemical Biology, MOE, College of Plant Protection, Fujian Agriculture and Forestry Univ, Fuzhou, China.
The interaction of microbial communities with host immunity has become one of the most explored research areas with significant implications for pest control strategies. It has been found that the gut microbiota plays substantial roles in immune response regulation and host-gut microbiome symbiosis, as well as in pathogen resistance and overall fitness in Tephritidae fruit flies that are major pests of agricultural importance. In this review, we discuss the modulation of immune responses of Tephritidae fruit flies by the gut microbiota with particular emphasis on the general interactions between microbiota and the immune system.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!