AI Article Synopsis
- A new method utilizing promoter hypermethylation of the SOCS-1 tumor suppressor gene shows promise for early diagnosis of gastric cancer.
- Researchers developed a fiber optic nanoplasmonic biosensor that can detect methylation in DNA without the need for PCR, utilizing PCR-free DNA from gastric tumor tissue and cell lines.
- The biosensor demonstrated high sensitivity with a detection limit of 0.81 fM for methylated DNA and provided results within 15 minutes, offering a more accurate alternative to traditional PCR methods for evaluating methylation status in cancer patients.
Article Abstract
A new innovative approach is essential for early and effective diagnosis of gastric cancer, using promoter hypermethylation of the tumor suppressor, SOCS-1, that is frequently inactivated in human cancers. We have developed an amplification-free fiber optic nanoplasmonic biosensor for detecting DNA methylation of the SOCS-1 human genome. The method is based on the fiber optic nanogold-linked sorbent assay of PCR-free DNA from human gastric tumor tissue and cell lines. We designed a specific DNA probe fabricated on the fiber core surface while the other probe is bioconjugated with gold nanoparticles in free form to allow percentage determination and differentiating the methylated and unmethylated cell lines, further demonstrating the SOCS-1 methylation occurs in cancer patients but not in normal cell lines. The observed detection limit is 0.81 fM for methylated DNA, and the detection time is within 15 min. In addition, our data were significantly correlated to the data obtained from PCR-based pyrosequencing, and yet with superior accuracy. Hence our results provide new insight to the quantitative evaluation of methylation status of the human genome and can act as an alternative to PCR with a great potential.
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| http://dx.doi.org/10.1016/j.bios.2022.114540 | DOI Listing |
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