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3 Biotech
February 2025
Centre for Research in Biotechnology for Agriculture (CEBAR), Universiti Malaya, 50603 Kuala Lumpur, Malaysia.
Unlabelled: Orphan genes (OGs), also known as lineage-specific genes, are species-specific genes that play a crucial role in species-specific adaptations to various stresses. Although OGs have been identified in several plant species, there is no information on OGs in banana genomes. This study aimed to systematically identify OGs in twelve banana (sub)species using comparative genomics.
View Article and Find Full Text PDFSci China Life Sci
January 2025
Frontiers Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China.
Since their discovery, CRISPR/Cas systems have significantly expanded the genetic toolbox, aiding in the exploration and enhanced production of natural products across various microbes. Among these, class 2 CRISPR/Cas systems are simpler and more broadly used, but they frequently fail to function effectively in many Streptomyces strains. In this study, we present an engineered class 1 type I CRISPR/Cas system derived from Streptomyces avermitilis, which enables efficient gene editing in phylogenetically distant Streptomyces strains.
View Article and Find Full Text PDFJ Sci Food Agric
January 2025
Research Center of Buckwheat Industry Technology, College of Life Science, Guizhou Normal University, Huaxi University Town, Gui'an New District, China.
Background: Tartary buckwheat (Fagopyrum tartaricum) is particularly vulnerable to pre-harvest sprouting (PHS) due to its extended flowering and fruiting cycle, especially during periods of prolonged rainfall. This susceptibility has significant adverse effects on yield, quality and post-harvest processing. In this study, a recombinant inbred lines (RILs) population (XJ-RILs) was developed from a cross between the PHS-susceptible Tartary buckwheat variety 'Xiaomiqiao' (female parent) and the highly PHS-resistant variety 'Jinqiaomai 2' (male parent).
View Article and Find Full Text PDFInt J Biol Macromol
January 2025
School of Landscape Architecture, Beijing Forestry University, Beijing 100083, China; Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, National Engineering Research Center for Floriculture, Engineering Research Center of Landscape Environment of Ministry of Education, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, China. Electronic address:
The GRAS gene family, is instrumental in a myriad of biological processes, including plant growth and development. Our findings revealed that Paeonia ludlowii (Stern & G.Taylor) D.
View Article and Find Full Text PDFMicrobiol Spectr
January 2025
Institute for Microbial Systems and Society, Faculty of Science, University of Regina, Regina, Saskatchewan, Canada.
Unlabelled: Antimicrobial resistance (AMR) is a global threat. The identification and characterization of novel resistance genes is integral to AMR surveillance. The (55) gene was originally identified through whole genome sequencing of macrolide-resistant strains of .
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