M13 bacteriophage (phage) are versatile, genetically tunable nanocarriers that have been recently adapted for use as diagnostic and therapeutic platforms. Applying p3 capsid chlorotoxin fusion with the "" circular single-stranded DNA (cssDNA) gene packaging system, we produced miniature chlorotoxin (CTX-) phage particles with a minimum length of 50 nm that can target intracranial orthotopic patient-derived GBM22 glioblastoma tumors in the brains of mice. Systemically administered indocyanine green conjugated CTX- phage accumulated in brain tumors, facilitating shortwave infrared detection. Furthermore, we show that our phage can carry cssDNA that are transcriptionally active when delivered to GBM22 glioma cells . The ability to modulate the capsid display, surface loading, phage length, and cssDNA gene content makes the recombinant M13 phage particle an ideal delivery platform.
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| http://dx.doi.org/10.1021/acsnano.1c08720 | DOI Listing |
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