PR-1-Like Protein as a Potential Target for the Identification of : An In Silico Approach.

BioTech (Basel)

Environmental Biotechnology Laboratory (EBL), Department of Biotechnology, University of the Western Cape, Cape Town 7535, South Africa.

Published: April 2021

remains one of the leading causes of economic losses and poor crop yields; its detection is strained due to its presentation in various morphological and physiological forms. This research work sought to identify novel biomarkers for the detection of using in silico approaches. Experimentally validated anti- antimicrobial peptides (AMPs) were used to construct a profile against . The performance and physicochemical parameters of these peptides were predicted. The gene for the receptor protein PR-1-like Protein, Fpr1, was identified and translated. The resulting protein model from the translation was then validated. The anti- AMPs and receptor protein 3-D structures were characterized, and their docking interaction analyses were carried out. The HMMER in silico tool identified novel anti- antimicrobial peptides with good performance in terms of accuracy, sensitivity, and specificity. These AMPs also displayed good physicochemical properties and bound with greater affinity to protein receptor PR-1-like Protein. The tendency of these AMPs to precisely detect PR-1-like Protein, Fpr1, would justify their use for the identification of the fungus. This study would enhance and facilitate the identification of to reduce problems associated with poor crop yield, economic losses, and decreased nutritional values of plants to keep up with the growing population.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9245465PMC
http://dx.doi.org/10.3390/biotech10020008DOI Listing

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