Reporter-expressing recombinant severe acute respiratory syndrome coronavirus 2 (rSARS-CoV-2) represents an excellent tool to understand the biology of and ease studying viral infections in vitro and in vivo. The broad range of applications of reporter-expressing recombinant viruses is due to the facilitated expression of fluorescence or bioluminescence readouts. In this chapter, we describe a detailed protocol on the generation of rSARS-CoV-2 expressing Venus, mCherry, and NLuc that represents a valid surrogate to track viral infections.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10243362 | PMC |
| http://dx.doi.org/10.1007/978-1-0716-2453-1_18 | DOI Listing |
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