Two new umbravirus-like associated RNAs (ulaRNAs) were found, respectively, in maize and Johnsongrass samples from Ecuador. The complete sequences consist of 3,053 and 3,025 nucleotides, respectively, and contain four open reading frames (ORFs). Their genome sequences were 58% identical to each other and 28 to 60% identical to the most closely related viruses. Phylogenetic analysis using full genome sequences and amino acid sequence of the RNA-dependent-RNA polymerase (RdRp) placed both sequences in a clade sharing the most recent common ancestor with ulaRNAs from sugarcane and maize, suggesting that they belong to a monophyletic grass-infecting lineage. Their terminal regions exhibit features common to umbraviruses and ulaRNAs.
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http://dx.doi.org/10.1007/s00705-022-05525-4 | DOI Listing |
PLoS Biol
April 2024
Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, Maryland, United States of America.
Viruses
December 2023
Skryabin Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky Prospect, 33, Build. 2, 119071 Moscow, Russia.
In this study, an analysis of the virome of 51 grapevines from the Don ampelographic collection named after Ya. I. Potapenko (Russia) was performed using high-throughput sequencing of total RNA.
View Article and Find Full Text PDFVirology
January 2024
Department of Plant Pathology, Kansas State University, Manhattan, KS, 66506, USA.
Umbravirus-like associated RNAs (ulaRNAs) are a new group of subviral RNAs associated with plants. Little is known about the biology of ulaRNAs. We recently reported wheat umbra-like virus (WULV) from Kansas fields.
View Article and Find Full Text PDFNucleic Acids Res
October 2023
Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD 20742, USA.
Plus-strand RNA viruses frequently employ -1 programmed ribosomal frameshifting (-1 PRF) to maximize their coding capacity. Ribosomes can frameshift at a slippery sequence if progression is impeded by a frameshift stimulating element (FSE), which is generally a stable, complex, dynamic structure with multiple conformations that contribute to the efficiency of -1 PRF. As FSE are usually analyzed separate from the viral genome, little is known about cis-acting long-distance interactions.
View Article and Find Full Text PDFArch Virol
October 2022
Department of Cell Biology and Molecular Genetics, University of Maryland College Park, College Park, MD, 20742, USA.
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