AI Article Synopsis
- Genetic screens are used to study biological pathways like membrane trafficking.
- CRISPR/Cas9 technology has improved the use of genetic screens in diploid models, particularly in cultured mammalian cells.
- The protocols provided are for creating custom CRISPR libraries, useful for both new model organisms without existing libraries and for focused research needs.
Article Abstract
Genetic screens are a classic approach to dissecting biological pathways including membrane trafficking. Clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 have enabled the utility of this approach in diploid models, including cultured mammalian cells. Here, we present detailed protocols for generating custom CRISPR libraries. These methods are useful for generating genome-wide libraries for new model organisms that lack an existing genome-wide library, and for generating smaller focused libraries.
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| http://dx.doi.org/10.1007/978-1-0716-2209-4_21 | DOI Listing |
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