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Novel MicroRNAs and their Functional Targets from and . | LitMetric
Background : Even though miRNAs play vital roles in developmental biology by regulating the translation of mRNAs, they are poorly studied in oomycetes, especially in the plant pathogen .Objective : The study aimed to predict and identify the putative miRNAs and their targets in and .Methods : The homology-based comparative method was used to identify the unique miRNA sequences in and with 148,689 EST and TSA sequences of these species. Secondary structure prediction of sRNAs for the 76 resultant sequences has been performed with the MFOLD tool, and their targets were predicted using psRNATarget.Results : Novel miRNAs, miR-8210 and miR-4968, were predicted from and , respectively, along with their structural features. The newly identified miRNAs were identified to play important roles in gene regulation, with few of their target genes predicted as transcription factors, tumor suppressor genes, stress-responsive genes, DNA repair genes, .Conclusion : The miRNAs and their targets identified have opened new interference and editing targets for the development of resistant crop varieties.
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