Background: Chickpea is one of Turkey's most significant legumes, and because of its high nutritional value, it is frequently preferred in human nourishment.Chloroplasts, which have their own genetic material, are organelles responsible for photosynthesis in plant cells and their genome contains non-trivial information about the molecular features and evolutionary process of plants.
Objective: Current study aimed at revealing complete chloroplast genome sequence of one of the wild type species, and comparing its genome with cultivated species, by using bioinformatics analysis tools. Except for , there has been no study on the chloroplast genome sequence of species.Therefore, we targeted to reveal the complete chloroplast genome sequence of wild type species, and compare the chloroplast genome of with the cultivated one
Methods: In this study, we sequenced the whole chloroplast genome of , one of the wild types of chickpea species, with the help Next Generation Sequencing platform and compared it with the chloroplast genome of the cultivated chickpea species, , by using online bioinformatics analysis tools.
Results: We determined the size of the chloroplast genome of as 124,804 bp and found that did not contain an inverted repeat region in its chloroplast genome. Comparative analysis of the chloroplast genome uncovered thirteen hotspot regions (psbA, matK, rpoB, rpoC1, rpoC2, psbI, psbK, accD, rps19, ycf2, ycf1, rps15, and ndhF) and seven of them (matK, accD, rps19, ycf1, ycf2, rps15 and ndhF) could potentially be used as strong molecular markers for species identification. It has been determined that was phylogenetically closer to cultivated chickpea as compared to the other species.
Conclusion: It is aimed that the data obtained from this study, which is the first study in which whole chloroplast genomes of wild chickpea species were sequenced, will guide researchers in future molecular, evolutionary, and genetic engineering studies with chickpea species.
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| http://dx.doi.org/10.2174/1389202923666220211113708 | DOI Listing |
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