AI Article Synopsis
- A new method for measuring salivary testosterone using a modified radioimmunoassay kit shows a strong correlation with serum free testosterone levels (r = 0.83, p < 0.01) across various concentrations.
- Precision testing for the salivary method indicates 11% within-batch and 18% between-batch variability, with an impressive recovery rate of 89%.
- This non-invasive approach provides a simpler, more affordable option for testosterone measurement compared to serum tests, reducing stress for participants.
Article Abstract
A direct non-extraction radioimmunoassay for salivary testosterone is described using a modified commercial kit procedure that is in use for total serum testosterone (T). Serum free testosterone was also measured by direct radioimmunoassay. A significant correlation (r = 0.83, p less than 0.01, n = 194) was obtained between salivary and serum free testosterone in matched serum and saliva samples over a wide range of concentrations. Within- and between-batch precision for the salivary testosterone method was 11% and 18%, respectively at a concentration of 170 pmol/l. Recovery of added T was 89% +/- 15% (mean +/- 2 SD) dilution of high samples showed parallelism. Salivary testosterone measured by direct radioimmunoassay offers a simple cheaper alternative to serum free testosterone measurement with the additional advantages of a stress-free non-invasive sampling procedure.
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| http://dx.doi.org/10.1016/0009-8981(87)90249-x | DOI Listing |
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