Phytotoxic effect of the insecticide imidacloprid in Phaseolus vulgaris L. plant and evaluation of its bioaccumulation and translocation by electrochemical methods.

The objective of this work is to study the toxicological effect of the imidacloprid (IMD) on common bean plants (Phaseolus vulgaris L) when used at high doses and its quantification by electrochemical method. Common bean plants were exposed to increasing concentrations of IMD and the different plant tissues were subjected to various analyses. The IMD detection in different tissues of the bean plant was performed after extraction on the metallic silver electrode using square wave voltammetry. The analytical and calibration parameters (Slope, correlation coefficient, linear range, detection limit and relative standard deviation) were calculated for the different plant tissues. The effect of different doses (5.0 × 10 to 5.0 × 10 mol L) of IMD was evaluated on germination, seedling (vigour, growth) and photosynthetic pigments in the bean plant. The results indicate that germination rate and seed vigour index reduced significantly (p ≤ 0.05) only in the applied concentrations above the recommended dose. A similar effect of IMD was observed on seedling development in term of roots length, plant length, number of leaves and number of nods. Concerning pigments content, chlorophyll a, b and total chlorophyll maximally decreased by 95.26%, 80.44% and 82.15% respectively at high applied dose. The bioaccumulation and translocation behaviour of IMD in bean plant was investigated, revealing that the IMD can be bioaccumulated in roots and can easily be translocated into stems and leaves.