AI Article Synopsis

  • - Dynamin is crucial for vesicle fission during endocytosis, with typical dynamics taking seconds, but neurons can achieve ultrafast endocytosis in just 50 ms.
  • - The study shows that a splice variant called Dynamin 1xA is pre-recruited to endocytic sites, aided by its interaction with Syndapin 1, leading to the formation of dynamic molecular condensates on the plasma membrane.
  • - Disruption of the interaction between Dynamin 1xA and Syndapin 1 prevents these condensates from forming, resulting in a significant slowdown of endocytosis, highlighting Syndapin 1's role as an adaptor that speeds up this process at synapses.

Article Abstract

Dynamin mediates fission of vesicles from the plasma membrane during endocytosis. Typically, dynamin is recruited from the cytosol to endocytic sites, requiring seconds to tens of seconds. However, ultrafast endocytosis in neurons internalizes vesicles as quickly as 50 ms during synaptic vesicle recycling. Here, we demonstrate that Dynamin 1 is pre-recruited to endocytic sites for ultrafast endocytosis. Specifically, Dynamin 1xA, a splice variant of Dynamin 1, interacts with Syndapin 1 to form molecular condensates on the plasma membrane. Single-particle tracking of Dynamin 1xA molecules confirms the liquid-like property of condensates in vivo. When Dynamin 1xA is mutated to disrupt its interaction with Syndapin 1, the condensates do not form, and consequently, ultrafast endocytosis slows down by 100-fold. Mechanistically, Syndapin 1 acts as an adaptor by binding the plasma membrane and stores Dynamin 1xA at endocytic sites. This cache bypasses the recruitment step and accelerates endocytosis at synapses.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9464723PMC
http://dx.doi.org/10.1016/j.neuron.2022.06.010DOI Listing

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