Context: F.C. How. (MO) (Rubiaceae) can strengthen bone function.
Objective: To examine the functional mechanism and effect of MO polysaccharides (MOPs) in rats with glucocorticoid-induced osteoporosis (GIOP).
Materials And Methods: Rats with GIOP were treated with 5, 15 or 45 mL/kg of MOP [ = 15 for each dose, intraperitoneal (i.p.) injection every other day for 8 weeks]. The body weight of rats and histomorphology of bone tissues were examined. Bone marrow mesenchymal stem cells (BMSCs)-derived exosomes (Exo) were collected and identified. Bone marrow-derived macrophages (BMMs) were induced to differentiate into osteoclasts and treated with BMSC-Exo for studies.
Results: MOP reduced the body weight (5, 15, or 45 mg/kg MOP vs. phosphate-buffered saline: 8%, 15% and 25%, < 0.01), elevated the bone volume to tissue volume (BV/TV), mean trabecular thickness (Tb.Th), mean trabecular number (Tb.N) and mean connectivity density (Conn.D) (40-86%, < 0.01), decreased the mean trabecular separation/spacing (Tb.Sp) (22-37%, < 0.01), increased the cortical bone continuity (35-90%, < 0.01) and elevated RUNX family transcription factor 2 and RANK levels (5-12%, < 0.01), but suppressed matrix metallopeptidase 9 and cathepsin K levels (9-20%, < 0.01) in femur tissues. BMSC-Exo from MOP-treated rats (MOP-Exo) suppressed osteoclastic differentiation and proliferation of BMMs. The downregulation of microRNA-101-3p (miR-101-3p) or the upregulation of prostaglandin-endoperoxide synthase 2 (PTGS2) blocked the functions of MOP-Exo.
Discussion And Conclusions: MOP inhibits osteoclastic differentiation and could potentially be used for osteoporosis management. This suppression may be enhanced by the upregulation of miR-101-3p or the inhibition of PTGS2.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9272931 | PMC |
| http://dx.doi.org/10.1080/13880209.2022.2093385 | DOI Listing |
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