Replication of the human genome initiates within broad zones of ∼150 kb. The extent to which firing of individual DNA replication origins within initiation zones is spatially stochastic or localised at defined sites remains a matter of debate. A thorough characterisation of the dynamic activation of origins within initiation zones is hampered by the lack of a high-resolution map of both their position and efficiency. To address this shortcoming, we describe a modification of initiation site sequencing (ini-seq), based on density substitution. Newly replicated DNA is rendered 'heavy-light' (HL) by incorporation of BrdUTP while unreplicated DNA remains 'light-light' (LL). Replicated HL-DNA is separated from unreplicated LL-DNA by equilibrium density gradient centrifugation, then both fractions are subjected to massive parallel sequencing. This allows precise mapping of 23,905 replication origins simultaneously with an assignment of a replication initiation efficiency score to each. We show that origin firing within early initiation zones is not randomly distributed. Rather, origins are arranged hierarchically with a set of very highly efficient origins marking zone boundaries. We propose that these origins explain much of the early firing activity arising within initiation zones, helping to unify the concept of replication initiation zones with the identification of discrete replication origin sites.
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http://dx.doi.org/10.1093/nar/gkac555 | DOI Listing |
Health Secur
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Jenna Mandel-Ricci, MPA, MPH, is Chief of Staff; both at the New York City Department of Health and Mental Hygiene, Long Island City, NY.
The New York City Department of Health and Mental Hygiene and the Greater New York Hospital Association held 3 workshops and 2 follow-up meetings with hospital emergency managers and colleagues to determine hospitals' response actions to a scenario of a 10-kiloton improvised nuclear device detonation. The scenario incorporated 3 zones of damage (moderate, light, and beyond damage zones) and covered the period of 0 to 72 hours postdetonation divided into 3 24-hour operational periods. The Joint Commission's critical emergency areas were used to determine the objectives and response actions that would be initiated.
View Article and Find Full Text PDFEvolution
December 2024
Museum für Naturkunde, Leibniz Institute for Evolution and Biodiversity, Center for Integrative Biodiversity Discovery, Invalidenstraße 43, 10115, Berlin, Germany.
In the more than 50 years since the initial conceptualization of the suture zone, little work has been done to take full advantage of the comparative capability of these geographic regions. During this time, great advances have been made in hybrid zone research that have provided invaluable insight in speciation and evolution. Hybrid zones have long been recognized to be "windows to the evolutionary process".
View Article and Find Full Text PDFPLoS One
December 2024
School of Architecture and Civil Engineering, Xi'an University of Science and Technology, Xi'an, China.
Understanding he impact of dry-wet and freeze-thaw cycles on the mechanical properties of unloaded damaged rock masses in reservoir bank slopes is crucial for revealing the deformation and failure mechanisms in artificially excavated slope rock masses within fluctuation zones. To address, the study focuses on unloaded damaged samples subjected to excavation disturbances, conducting various cycles of dry-wet and freeze-thaw treatment along with uniaxial and triaxial re-loading tests. A damage statistical constitutive model was established based on the experimental results and validated using numerical simulation methods.
View Article and Find Full Text PDFFront Health Serv
December 2024
Health Research and Technology Transfer Directorate, South Ethiopia Region Public Health Institute, Jinka, Ethiopia.
Background: Women's empowerment is one of the fundamental issues for attaining sustainable development goals crossing multiple dimensions. In Ethiopia, Women's Development Network (WDN), a network of women, was established in 2010 with development aims. Ensuring women's empowerment critically needs collective efforts of platforms such as WDN.
View Article and Find Full Text PDFCan Respir J
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Department of Radiation Oncology, Tangdu Hospital, The Second Affiliated Hospital of Air Force Medical University, Xi'an, China.
Thymoma is a common malignancy with low incidence, and pleural metastases are common pattern of recurrence. It is necessary that the spatial location of pleural metastatic lesions be analyzed. This study aimed to analyze the spatial distribution patterns of pleural dissemination in patients with thymoma and evaluate the variables that influence the survival of pleural metastasis in this population.
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