This study proposes a new method to promote the granulation process while accelerating the degradation efficiency of nutrients. The new strategy could involve preparing Fe-loaded activated carbon (FAC) before start-up of granular cultivation and then cultivating the process of aerobic granular sludge (AGS) with such materials. In addition, this experiment could further comprehend how the preparation and characteristics of FAC affect the formation and properties of AGS. The conclusions showed that compared with the control, FAC enhanced the sedimentation performance and significant removal efficiency. Meanwhile, the values of protein (PN) and polysaccharide (PS) also increased significantly in the addition of FAC, indicating the production of substances were induced by FAC. Molecular biology methods indicated that the rapid production of granulation and removal of nutrients were considered as the abundance of various microbes and denitrifying bacteria at the addition of FAC. This research showed that the presence of FAC is a useful strategy for the initiation of sludge particle formation to promote the treatment of wastewater, containing COD and NH at about 150-100 and 30 mg L.
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http://dx.doi.org/10.1016/j.chemosphere.2022.135532 | DOI Listing |
Bioresour Technol
January 2025
School of Resources and Environment, Hubei Key Laboratory of Regional Development and Environmental Response, Hubei University, Wuhan 430062, China.
In aerobic granular sludge (AGS) system, N-acyl homoserine lactones (AHLs) can effectively regulate the community structure and control filamentous bulking. It would be economically feasible to make mature granules into AHLs-rich AGS extract (AE) to replace synthesized AHLs. In this study, two SBRs were run in a fully aerobic environment and a short cycle (4 h) to culture AGS: R1 with AE adding; R2 served as control.
View Article and Find Full Text PDFBioresour Technol
January 2025
School of Urban Construction, Wuhan University of Science and Technology, Wuhan 430065, China.
Ammonia-oxidizing bacteria (AOB) sourced from an aerobic granular sludge (AGS) process were rapidly enriched by progressively increasing ammonia nitrogen (NH-N) loads, achieving a Nitrosomonas abundance of 20.7 % and a nitrite accumulation rate exceeding 80 %. Mycelial pellets formed by Cladosporium, isolated from the same AGS system, provided a porous surface structure for the immobilization of the enriched AOB, creating mycelial pellet/AOB composites.
View Article and Find Full Text PDFWater Res
December 2024
Eawag: Swiss Federal Institute of Aquatic Science and Technology, Überlandstrasse 133, 8600, Dübendorf, Switzerland. Electronic address:
Aerobic granular sludge (AGS) is usually considered to be a biofilm system consisting of granules only, although practical experience suggests that flocs and granules of various sizes co-exist. This study thus focused on understanding the contribution of flocs and granules of various sizes to nitrification in a full-scale AGS-based wastewater treatment plant (WWTP) operated as a sequencing batch reactor (SBR). The size distribution in terms of total suspended solids (TSS) and the distribution of the nitrifying communities and activities were monitored over 14 months.
View Article and Find Full Text PDFEnviron Res
January 2025
National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Beijing University of Technology, Beijing, 100124, China. Electronic address:
Dinotefuran (DIN) is toxic to non-target organisms and accelerates the evolution of antibiotic resistance, which poses a problem for the stable operation of the activated sludge process in wastewater treatment plants (WWTPs). However, the emergence and the transfer mechanism of antibiotic resistance genes (ARGs) in activated sludge systems under DIN stress remains unclear. Thus, in the study, the potential impact of DIN on ARGs and virulence factor genes (VFGs) in aerobic granular sludge (AGS) was investigated in depth using metagenomic binning and functional modules.
View Article and Find Full Text PDFJ Environ Manage
January 2025
Department of Environmental Science and Engineering, Zhejiang Ocean University, No.1 Haida South Road, Zhoushan, 316022, PR China; Zhejiang Provincial Key Laboratory of Petrochemical Environmental Pollution Control, Zhoushan, 316022, PR China. Electronic address:
In order to study the resistance mechanisms of biofilm and granular sludge to various dissolved oxygen (DO) exposures in anaerobic ammonium oxidation (anammox) process, a biofilm - granular sludge anammox reactor was established and operated. Experimental results showed that DO levels of ≤0.41 mg L hardly affected the total nitrogen removal efficiency (TNRE).
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