This study aimed to investigate the presence of and genes in coagulase negative (CNS) species isolated from bovine mastitis in smallholder dairy farms by using PCR. A total of 602 mammary quarter milk samples belong to 170 cows with mastitis were used. Identification of species was achieved by using the commercial Gram-positive identification kit and a total of 52 (8.6%) CNS species were isolated. The most frequently isolated species was (n = 15, 28.8%), followed by (n = 12, 23.1%), (n = 8, 15.4%), (n = 5, 9.6%), (n = 4, 7.7%), (n = 4, 7.7%), (n = 2, 3.8%) and (n = 2, 3.8%). The gene positivity was found in the 13 (25%) of strains. Of the strains carrying gene, eight also harbored the gene. A total of gene positivity was found as 30.8% (n = 16) in 52 CNS species. In conclusion, the present study showed that CNS isolated from cows with mastitis may be reservoir of and genes. To our knowledge, this is the first study showing the presence of and genes in CNS species isolated from bovine with mastitis in the smallholder dairy farms in Turkey.
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http://dx.doi.org/10.1080/10495398.2022.2094802 | DOI Listing |
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