[Aerobic Granulation Stability and Microbial Diversity of Filamentous Bulking Sludge].

Huan Jing Ke Xue

National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Faculty of Environment and Life, Beijing University of Technology, Beijing 100124, China.

Published: July 2022

AI Article Synopsis

  • Filamentous bacteria contribute to sludge bulking in wastewater treatment but also aid in forming stable sludge particles, crucial for effective treatments.
  • Research shows that bulking sludge forms granules faster and larger than flocculated sludge, with bulking achieving maturity in just 20 days compared to 40 days for flocculated sludge.
  • The presence of specific hydrophobic bacteria boosted rapid granulation and enhanced overall removal rates for pollutants like COD, NH-N, and TN in bulking sludge compared to flocculated sludge.

Article Abstract

Filamentous bacteria, as one of the common bacteria types in wastewater biological treatment, are considered to be the main factor to induce sludge bulking. However, because of its special filamentous shape, it plays a crucial role in the formation of sludge particles. Taking filamentous bulking sludge as the research object, the effect of filamentous bacteria on the sludge granulation process and maintaining the stability of sludge granules was studied, and the microbial diversity of the sludge system was analyzed. Filamentous bulking sludge (SVI=241.56 mL·g) and flocculated sludge (SVI=64.22 mL·g) were respectively inoculated to carry out granulation culture. The results showed that the time of particle appearance of bulking sludge and flocculated sludge was 20 days and 40 days, respectively; the mature particle sizes were 650 μm and 700 μm, respectively; and the granulation time of bulking sludge was only half that of flocculated sludge. After adding the anoxic zone, the granules were broken to differing degrees, but the SV/SV value of mature granules recovered to 1 after short-term fluctuation, and the stability of the mature granules was stronger. The analysis of microbial community structure showed that the relative abundance of , , and increased from 0.05%, 0.01%, and 0.01% to 4.09%, 3.15%, and 1.12%. The existence and accumulations of these hydrophobic bacteria were important for rapid granulation. The removal rates of COD, NH-N, and TN were 94%, 99%, and 35% and 92%, 97%, and 30%, respectively, in SBR of bulking sludge and SBR of flocculated sludge, and the removal rates of TP were 60% and 30%, respectively.

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http://dx.doi.org/10.13227/j.hjkx.202110105DOI Listing

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