AI Article Synopsis
- The SARS-CoV-2 pandemic has spurred interest in understanding coronaviruses and developing relevant reagents for research and treatment.
- The study details the biochemical evaluation of monoclonal antibodies that were synthetically created and identified using phage-display techniques against SARS-CoV-2 proteins.
- Binding kinetics of these antibodies were assessed using various methods, ensuring their effectiveness and reliability through comparisons with control proteins and the purified virus.
Article Abstract
SARS-CoV-2 pandemic opens up the curiosity of understanding the coronavirus. This demand for the development of the regent, which can be used for academic and therapeutic applications. The present data provide the biochemical characterization of synthetically developed monoclonal antibodies for the SARS-CoV-2 proteins. The antibodies from phage-displayed antibody libraries were selected with the SARS-CoV-2 proteins immobilized in microwell plates. The clones which bind to the antigen in Fab-phage ELISA were selected, and a two-point competitive phage ELISA was performed. Antibodies binding kinetic of IgGs for SARS-CoV2 proteins further carried with B.L.I. Systematic analysis of binding with different control proteins and purified SARS-CoV-2 ensured the robustness of the antibodies.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9232259 | PMC |
| http://dx.doi.org/10.1016/j.dib.2022.108415 | DOI Listing |
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