AI Article Synopsis
- Arl5b is a small G protein located on the trans-Golgi network (TGN) that plays a crucial role in regulating the transport of endosomes to the TGN.
- Researchers used various techniques to identify Arl5b's interactive partners and nearby proteins, discovering 22 Golgi proteins, primarily composed of TGN-localised small G proteins like Rabs, Arfs, and Arls.
- The study highlights the importance of Arl5b in the recruitment of scaffold/tethering factors such as ACBD3, indicating a complex network of small G proteins that organizes the TGN membrane.
Article Abstract
The small G protein Arl5b is localised on the trans-Golgi network (TGN) and regulates endosomes-to-TGN transport. Here, we combined in vivo and in vitro techniques to map the interactive partners and near neighbours of Arl5b at the TGN, using constitutively active, membrane-bound Arl5b(Q70L)-GFP in stably expressing HeLa cells, and the proximity labelling techniques BioID and APEX2 in parallel with GFP-Trap pull down. From MS analysis, 22 Golgi proteins were identified; 50% were TGN-localised Rabs, Arfs and Arls. The scaffold/tethering factors ACBD3 (GCP60) and PIST (GOPC) were also identified, and we show that Arl5b is required for TGN recruitment of ACBD3. Overall, the combination of in vivo labelling and direct pull downs indicates a highly organised complex of small G proteins on TGN membranes.
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| http://dx.doi.org/10.1002/1873-3468.14443 | DOI Listing |
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