The biochemical properties of α-1,3-galactosyltransferase WciN from serotype 6B were systemically characterized with the chemically synthesized Glcα-PP-(CH)-OPh as an acceptor substrate. The site-directed mutation of D38 and A150 residues of WciN was further investigated, and the enzymatic activities of those WciN mutants revealed that A150 residue was the pivotal residue responsible for nucleotide donor recognition and the single-site mutation could completely cause pneumococcus serotype switch. Using WciN and WciN mutants as useful tool enzymes, the disaccharides Galα1,3Glcα-PP-(CH)-OPh and Glcα1,3Glcα-PP-(CH)-OPh were successfully prepared in multi-milligram scale in high yields.
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