AI Article Synopsis
- Recent advancements in genome sequencing have identified various yeasts with unique physiological traits, but the absence of effective expression tools limits their application in research and industry.
- A new universal expression system (SES) has been developed, enabling gene expression control and genetic manipulation across a wide range of fungal species.
- The article outlines three methods for DNA transformation and high-level gene expression using the SES system in yeast species that have little existing knowledge in genetic modifications.
Article Abstract
The current progress in sequencing of genomes and characterization of new species provides an increasing list of yeasts that show interesting physiological properties; however, the lack of expression tools for these new hosts is prohibiting their broader use in research or industry. Recently, we developed a universal expression system (SES) functional in broad spectrum of fungal species, which represent a solution for feasible gene expression control and genetic manipulation in these novel hosts. Here, we describe three example approaches for DNA transformation and high-level heterologous gene expression, using the SES system, in three yeast species, where minimal knowledge or prior experience in genetic modifications is available.
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| http://dx.doi.org/10.1007/978-1-0716-2399-2_13 | DOI Listing |
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