Due to limited detection sensitivity and contrast limitation, imaging substrates with Xe MRI in living cells is still a challenge. Here, we present an effective protocol to detect and image substrates in human lung cancer cells A549 with hyperpolarized Xe MRI. This protocol was optimized for a cryptophane-based probe sensitive to biothiols and can be expanded to other Xe-based probes to detect potential biomarkers in other mammalian cells. For complete details on the use and execution of this protocol, please refer to Zeng et al. (2021).
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9249822 | PMC |
| http://dx.doi.org/10.1016/j.xpro.2022.101499 | DOI Listing |
Publication Analysis
Top Keywords
living cells
8
hyperpolarized mri
8
protocol
4
protocol detecting
4
detecting substrates
4
substrates living
4
cells
4
cells targeted
4
targeted molecular
4
molecular probes
4
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!