P2X7 receptors are expressed in different circulating immune cells. Depending on the cell in which they are expressed, their function could vary from activation of the leucine-rich repeat receptor pyrin domain containing 3 (NLRP3) inflammasome or the shedding of different surface receptors. The P2X7 receptor has been involved in the evolution of several inflammatory diseases, and thus its characterization in blood samples is important to investigate its role in human diseases. In this chapter, we present different methods to detect the presence and the activation of P2X7 receptors in whole blood samples. These methods could be used to evaluate the degree of P2X7 receptor activation in blood samples from patients suffering different chronic inflammatory diseases.
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