AI Article Synopsis
- This study presents a new imaging technique called polarization sensitive optical coherence tomography (PS-OCT) that allows for long-depth-of-focus imaging through advanced computational methods.
- The technique combines phase-sensitive refocusing based on the Fresnel diffraction model with Jones-matrix based PS-OCT, capturing four complex images for different polarization channels.
- The effectiveness of this method was tested on various samples, including zebrafish and porcine muscle, showing that computational refocusing minimizes polarization artifacts that usually distort image quality.
Article Abstract
Here we demonstrate a long-depth-of-focus imaging method using polarization sensitive optical coherence tomography (PS-OCT). This method involves a combination of Fresnel-diffraction-model-based phase sensitive computational refocusing and Jones-matrix based PS-OCT (JM-OCT). JM-OCT measures four complex OCT images corresponding to four polarization channels. These OCT images are computationally refocused as preserving the mutual phase consistency. This method is validated using a static phantom, postmortem zebrafish, and porcine muscle samples. All the samples demonstrated successful computationally-refocused birefringence and degree-of-polarization-uniformity (DOPU) images. We found that defocusing induces polarization artifacts, i.e., incorrectly high birefringence values and low DOPU values, which are substantially mitigated by computational refocusing.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9203103 | PMC |
| http://dx.doi.org/10.1364/BOE.454975 | DOI Listing |
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