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Human Bronchial Epithelial Cell Culture Models for Cigarette Smoke and Vaping Studies. | LitMetric

Human Bronchial Epithelial Cell Culture Models for Cigarette Smoke and Vaping Studies.

Methods Mol Biol

Department of Medicine, National Jewish Health, Denver, CO, USA.

Published: July 2022

AI Article Synopsis

Article Abstract

Despite the continuing public health efforts to stop or reduce smoking, cigarette smoke use remains popular in the youth and adult population. A recent surge in the use of electronic cigarette and vaping products has created another major health challenge in public health. There is an urgent need to use physiologically relevant models to study the health effect of smoking or vaping in human subjects. Airway diseases such as bronchitis (Landman et al., CMAJ 191:E1321-E1331, 2019; Goniewicz, et al. Harm Reduct J 17:91, 2020; Xie et al., JAMA Netw Open 3:e2020816, 2020) have been described in people who smoke, vape, or both. Here, we will describe methods to collect, expand, and culture human airway epithelial cells from endobronchial brushings and expose these cells cultured at the air-liquid interface to cigarette smoke or electronic cigarette vapor.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9306142PMC
http://dx.doi.org/10.1007/978-1-0716-2364-0_10DOI Listing

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