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Investigating Mitochondrial Ca Dynamics in Isolated Mitochondria and Intact Cells: Application of Fluorescent Dyes and Genetic Reporters. | LitMetric
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Investigating Mitochondrial Ca Dynamics in Isolated Mitochondria and Intact Cells: Application of Fluorescent Dyes and Genetic Reporters.

Gauri Bhosale Michael R Duchen

Methods Mol Biol

Department of Cell and Developmental Biology and Consortium for Mitochondrial Research, UCL, London, UK.

Published: July 2022

Mitochondrial Ca buffering is a hallmark of eukaryotic cellular physiology, contributing to the spatiotemporal shaping of the cytosolic Ca signals and regulation of mitochondrial bioenergetics. Often, this process is altered in a pathological context; therefore, it can be scrutinized experimentally for therapeutic intervention. In this chapter, we describe fluorescence and bioluminescence measurement of mitochondrial Ca in both isolated mitochondria and intact cells.

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http://dx.doi.org/10.1007/978-1-0716-2309-1_23DOI Listing

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