AI Article Synopsis
- The ubiquinone (Q) pool is a critical component in the mitochondrial electron transport chain, acting as a convergence point for electrons from various respiratory enzymes.
- The redox state of the Q pool is closely linked to the efficiency of electron flow through the ETC, making it an important indicator of mitochondrial and cellular metabolism.
- This study details a method for measuring both the respiratory rates of isolated mouse heart mitochondria and the redox state of the Q pool using a specially designed Clark-type oxygen electrode combined with a Q electrode.
Article Abstract
The ubiquinone (Q) pool represents a node in the mitochondrial electron transport chain (ETC) onto which the electrons of all respiratory dehydrogenases converge. The redox state of the Q pool correlates closely with the electron flux through the ETC and is thus a parameter of great metabolic value for both the mitochondrial and cellular metabolism. Here, we describe the simultaneous measurement of respiratory rates of isolated mouse heart mitochondria and the redox state of their Q pool using a custom-made combination of a Clark-type oxygen electrode and a Q electrode.
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| http://dx.doi.org/10.1007/978-1-0716-2309-1_19 | DOI Listing |
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